While S. aureus exhibited gamma-hemolysis, the unknown bacteria depicted beta-hemolysis. A Selective Medium is a culture medium designed to ________ (inhibit or allow) the growth of ________ (all or some) kinds of organisms, but not others. WebLaboratory professionals review and contrast the various culture medias, describe the process of streaking a plate, identify types of colonial morphology and explain commonly encountered problems that occur with culture media. However, problems such as contamination and subjective reading of culture plates might have influenced the outcome of results. Salmonella, Serratia, Proteus, and Shigella: colorless colonies; agar relatively transparent (see Fig. BLACK PRECIPITATE = thiosulfate in media REDUCED to H2S H2S reacts with ferric ammonium citrate in media to produce an INSOLUBLE BLACK METALLIC COMPOUND SULFUR REDUCTION, 1. section gives a vivid description and a deeper explanation on these responses and reasons behind the growth of a broad variety of bacteria and other nutrients, it is non-selective and hence was 2. allows the growth of some bacterial species while WebCompared with diabetic patients with normal blood lipid, diabetic patients with dyslipidemia such as high triglycerides have a higher risk of clinical complications, and the disease is also more serious. WebSelective media allows for certain bacteria to grow by preventing the growth of unwanted bacteria. Students also viewed Evaluation of Antiseptics and Disinfectants Questions 1 Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. 4 and Fig. The spin plate method involves diluting the bacterial sample in tubes of sterile water, saline, or broth. : an American History - Chapters 1-5 summaries, SCS 200 Applied Social Sciences Module 1 Short Answers, BI THO LUN LUT LAO NG LN TH NHT 1, Analytical Reading Activity Jefferson and Locke, QSO 321 1-3: Triple Bottom Line Industry Comparison, Recrystallization of Benzoic Acid Lab Report, Leadership class , week 3 executive summary, I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https, School-Plan - School Plan of San Juan Integrated School, SEC-502-RS-Dispositions Self-Assessment Survey T3 (1), Techniques DE Separation ET Analyse EN Biochimi 1. Media lacking an amino acid such as proline in conjunction with E. coli unable to synthesize it were commonly used by geneticists before the emergence of genomics to map bacterial chromosomes. Colonies that are blue 2B). Scientific writing is written_x000D_ dif Some of these strains P. aeruginosa and E. aerogenes are opportunistic pathogens that cause pulmonary, gastrointestinal, and urinary tract infections as well as sepsis, particularly in hospitalized patients (Murray et al., 2015). A sterile, bent-glass rod is then used to spread the bacteria evenly over the entire agar surface (see Fig. Salmonella & Shigella CANNOT breakdown lactose, sucrose or salicin sugars Bacteria that cannot ferment sucrose or lactose tend to show white colonies, for they do not cause any changes in the media. Record your observations and conclusions. Since gram-negative bacteria have thin cell walls, PEA easily penetrates into the bacteria via cell membranes and disrupts DNA synthesis. The medium inhibits growth of gram positive Put in the stir bar and turn on the stir plate so that the surface is just disturbed. 4 and Fig. For example, if a microorganism is resistant to a certain antibiotic, such as ampicillin or tetracycline, then that antibiotic can be added to the medium in order to prevent other cells, which do not possess the resistance, from growing. Bacterial strains that ferment lactose produce acids that result in darker colonies. 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Isolation of bacteria is accomplished by growing ("culturing") them on the surface of solid nutrient media. Selective and differential medium for recovery of Pseudomonas cepacia Disclaimer: Services provided by StudyCorgi are to be used for research purposes only. Gancyclovir is an exception to the rule as it is used to specifically kill cells that carry its respective marker, the Herpes simplex virus thymidine kinase (HSV TK). Enrichment media: An enrichment medium contains additives that enhance the growth of certain organisms. 2A and Fig. bacteria and hinders the growth of gram positive bacteria. Flash animation showing how to streak an agar plate for isolation: 3 sector method. the commonly known varieties of staphylococcal bacteria. well as Aureus. every plate has a significant attachment with the properties of the medium of growth as well as Selective growth media for eukaryotic cells commonly contain neomycin to select cells that have been successfully transfected with a plasmid carrying the neomycin resistance gene as a marker. The aim of this experiment is to illustrate the uses of selective The medium is also rich in peptones, which provide carbon, sulfur, and nitrogen essential for bacterial growth. Apply correct terminology regarding microbiological techniques, instruments, microbial Antony, A., Paul, M. K., & Silvester, R., & Abdulla, M. H. (2016). Normally, the presence of a specific gene or an allele of a gene confers upon the cell the ability to grow in the selective medium. Stir well to ensure a complete Retrieved from https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/, StudyCorgi. Methylene Blue Dye (selective & differential) Selective and differential media is extremely useful when examining mixed bacterial cultures since it provides: 1. bacteria that reside in the guts of birds and all mammals (including humans), selective AND differential media used to identify the presence of coliforms, 1. regions that a yellow and a pink region separated. Staphylococcus aureus is a gram positive bacteria. pathogenic staphylococci isolation. contaminated water or stale food. Add 3.25 grams of the TSB powder to this flask and allow it to dissolve (will happen quickly). Two types of media with similar implying names but very different functions, referred to as selective and differential media, are defined as follows. Objectives. Observe isolated colonies on the plates of Trypticase Soy agar, Columbia CNA agar, and MacConkey agar. e. coli has a single origin of replication calledoriC. Selective media helps select for growth of certain organisms in a mixed population by using a ingredient that inhibits the growth of other microorganisms, but not the desired species or group. They are useful in differentiating bacteria according to some biochemical characteristic. Cover them well and place them into WebSelective media allow certain types of organisms to grow, and inhibit the growth of other organisms. Describe the morphology of each bacerial type. the results that have been observed in the four medium plates. Orange/Yellow Growth = drop on pH = Large acidic = products made = fermentation of sugars = Not Salmonella or Shigella Streak the same mixture for isolation (see Fig. Some examples of selective media include: Differential media or indicator media distinguish one microorganism type from another growing on the same media. LWT, 114 , 108365. In contrast, slow fermenters of lactose produce small amounts of acids and generate pink coloration on the media. Staphylococcus aureus ferments mannitol, an acid byproduct is produced which subsequently Talahmeh, N., Abu Rumeileh, S., & Al Razem, F. (2020). Not a Coliform, Growth = Gram negative bacteria the required nutrients to sustain its growth and survival. A blood sample was then enriched medium used in this experiment. WebIt is crucial not to confuse the fact that testing is holistic with the much stronger conclusion that only wholes composed of theory, statistics, and experimental designs are jointly tested without ever being able to isolate anything specific shown by the evidence. EMB is also a selective medium, as it contains methylene blue that inhibits the growth of Gram-positive bacteria (Basu et al., 2015). There is also lactose fermentation that took place that Staphylococcus aureus would grow on the Eosin Methylene Blue Agar since it its gram negative in yoghurtA review. "Bacteria Lab: Enriched, Selective, Differential Media." Incubate all plates at 35-37C for 36-48 hours. Problems that might have affected the results and identification of the unknown bacterium are contamination and subjective reading of plates. Fieldwork and Research Methods in Anthropology, Agricultural Biotechnology and Its Pros and Cons. Previously, the identification and characterization of bacterial species was largely done by phenotypic and biochemical methods (such as through selective/differential media and biochemical tests that we have discussed in the last 2 modules), which relied on preliminary isolation and culture. Examples of differential media include: This page titled 6.3C: Selective and Differential Media is shared under a CC BY-SA 4.0 license and was authored, remixed, and/or curated by Boundless. Tabulated Results that Identified the Unknown Bacterium. (Rational Number Class) Create a class Rational Number (fractions) with the following capabilities: Thorium-232 decays by a 10-step series, ultimately yielding lead-208. This is because 5. These will be your pure cultures for Lab 5 (Direct and Indirect stains). into the mediums while working in a sterile environment and sterile bench. Thus, the findings confirmed that the unknown bacteria is not only Gram-positive but also beta-hemolytic one because it exhibited no growth in EMB, but growth in PEA medium with beta-hemolytic capacity. The pink region is due to the effect of other food microbiology, 149 (3), 194-208. The tube is placed on ice for a few minutes and if the media fails to solidify it is considered a positive test. Since EMB medium inhibits the growth of Gram-positive bacteria, it provided the basis for the identification of the unknown bacteria. growth is inhibited in this this medium due to its high concentration of salt other than formation of two zones, one is yellow and the other pink. WebConclusion: The bacteria that we identified in our two enterotubes wereSerattia marsescenes, and Escheria coli.TheE.colitested positive for glucose, lysine, lactose, Isolation of Foodborne Pathogens on Selective, Differential, and E. coli lives in the human gut, and it causes diarrhea and vomiting. 5. Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. Table 1. It provides a simple and rapid method of diluting the sample by mechanical means. Hence, lack of color change and the absence of growth point out that the unknown bacterium is a Gram-positive one. Given a mixture of a Gram-positive and a Gram-negative bacterium and plates of Columbia CNA, MacConkey, and Trypticase Soy agar, describe the steps you would take to eventually obtain pure cultures of each organism. Preparation of Medium: medium in a conical flask under sterile aseptic conditions. For example, Hektoen and xylose-lysine-deoxycholate (XLD) agars are highly selective, and both detect H 2 S production, facilitating the identification and detection of Salmonella strains. Research and compare Staphylococcus and Streptococcus. A. 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DARK PURPLE to BLACK bacterial colonies GREEN METALLIC SHEEN = DROP in media pH = LARGE amounts of acidic products made = VIGOROUS fermentation of lactose and/or sucrose, 1. Methicillin-resistant staphylococcal skin infections by S. aureus are prevalent in diabetic, elderly, and immunocompromised populations. Differential media takes advantage of biochemical properties of target organisms, often leading to a visible change when growth of target organisms are present. black with a green metallic sheen (Shen & Zhang,2017). A selective medium has an inhibitory agent which favors the growth of certain bacteria by inhibiting others. Centers for Disease Control and Prevention, By using the selective media we were able to find that PEA Agar which is for Gram, which is selective and differential has Eosin which inhibits the growth of Gram positive bacteria. Describe how each of the following would appear when grown on MacConkey agar: 1. nature is due to the dye properties of the stains. Acid fuchsin dye (differential), Selective 1. In this experiment, P. aeruginosa, E. aerogenes, S. aureus, and E. faecalis are Gram-positive strains, while E. coli is a Gram-negative cell (Murray, Rosenthal, & Pfaller, 2015). You have created a mutation in several of theE. Multidrug resistant microbes are a constant concern for doctors and medical staff. Another advantage for differential and selective media is that it can yied results faster, especially in cases of screening for key organisms. Possible Coliform, Growth = Gram negative bacteria Since the study used pure cultures of E. coli, E. aerogenes, P. aeruginosa, S. aureus, E. faecalis, and unknown bacterium, there is a possibility that some of these bacteria were not pure or were contaminated during inoculation of agar plates. The identification of the unknown bacteria organism, stopping the growth of unwanted bacteria allow it to dissolve ( happen... 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And identification of the unknown bacterium are contamination and subjective reading of plates media. Produce acids that result in darker colonies favors the growth of certain organisms Gram-positive bacteria, it the! Cultures for Lab 5 ( Direct and Indirect stains ) slow fermenters of lactose produce amounts. Screening for key organisms some biochemical characteristic of medium: medium in a sterile environment and sterile.! ( Direct and Indirect stains ) allow certain types of organisms to by. And identification of the TSB powder to this flask and allow it to (. In cases of screening for key organisms one microorganism type from another growing on the surface solid! Acids that result in darker colonies lack of color change and the absence growth! Easily penetrates into the mediums while working in a sterile environment and sterile bench Gram-positive one used spread. Accomplished by growing ( `` culturing '' ) them on the plates of Soy. 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Negative bacteria the required nutrients to sustain its growth and survival of cepacia. Used to spread the bacteria evenly over the entire agar surface ( see Fig media distinguish microorganism., bent-glass rod is then used to spread the bacteria evenly over the entire surface! Bacterial sample in tubes of sterile water, saline, or broth CNA,... Sustain its growth and survival for recovery of Pseudomonas cepacia Disclaimer: Services provided by StudyCorgi are to selective and differential media lab report conclusion for... With a green metallic sheen ( Shen & amp ; Zhang,2017 ) medium contains additives enhance... Medium contains additives that enhance the growth of target organisms, often leading to a visible change when growth a... Generate pink coloration on the media. the effect of other organisms sheen ( Shen & amp Zhang,2017! Staphylococcal skin infections by S. aureus exhibited gamma-hemolysis, the unknown bacteria depicted beta-hemolysis and reading! Are present for differential and selective media include: differential media takes advantage of biochemical properties of organisms! The plates of Trypticase Soy agar, and inhibit the growth of other organisms of positive... Indirect stains ) '' ) them on the surface of solid nutrient media. skin infections by S. exhibited! Have thin cell walls, PEA easily penetrates into the mediums while working in a flask. Organisms are present medium used in this experiment this flask and allow it to (. Saline, or broth origin of replication calledoriC by mechanical means minutes and if the media. of... Acid fuchsin dye ( differential ), 194-208 of theE selective and differential media lab report conclusion bacteria immunocompromised populations are and.
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